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. Author manuscript; available in PMC: 2016 Dec 29.
Published in final edited form as: ACS Chem Biol. 2016 Jun 13;11(8):2206–2215. doi: 10.1021/acschembio.6b00145

Figure 3.

Figure 3

Characterization of TAR RNA binding affinity and selectivity. (A) ELISA analysis of selected 6th generation TAR-Binding Proteins (TPBs). (B) U1A scaffold highlighting mutations identified in the tightest TBPs: TBP 6.8 and TBP 6.9. (C) ELISA data showing binding between TAR RNA and TBP 6.6 (blue) or TBP 6.7 (black). (D) SPR data for the binding interactions between TBP 6.6 and TAR RNA (left column, top row); TBP 6.7 and TAR RNA (left column, middle row); U1A and TAR RNA (left column, bottom row); TBP 6.6 and U1hpII RNA (right column, top row); TBP 6.7 and U1hpII RNA (right column, middle row); U1A and U1hpII RNA (right column, bottom row). (E) Sequence and secondary structures of TAR-derived mutants used to characterize the requirements for TAR recognition by TBP 6.6 and TBP 6.7. (F) ELISA data showing binding between TBP 6.6 or TBP 6.7 and TAR mutants hp1-6 and BIV TAR.