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. Author manuscript; available in PMC: 2016 Dec 29.
Published in final edited form as: Cell Rep. 2016 Nov 15;17(8):2004–2014. doi: 10.1016/j.celrep.2016.10.073

Figure 2. CAPS1 RNA Editing Promotes Secretion of Noradrenaline from PC12 Cells.

Figure 2

(A) Stable cell lines expressing either unedited or edited mCherry-mCAPS1 were established using shRNA_All-8 PC12 cells, in which endogenous CAPS1 was strongly knocked down (see Figure S2). The expression of exogenous mCherry-mCAPS1 was confirmed by western blot analysis using an anti-CAPS1 antibody in two lines (designated as 1 and 2) for the unedited and two for the edited forms. The expression of GAPDH is shown as a reference.

(B) The percentage efficiency of noradrenaline (NA) release from the cells expressing either unedited or edited mCherry-mCAPS1 was measured at 15, 30, and 60 min after stimulation with high K+ buffer. Values are displayed as the mean ± SEM (ten trials for each line; unpaired t test, *p < 0.05). n.s., no significance; NS, no stimulation.