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. Author manuscript; available in PMC: 2016 Dec 29.
Published in final edited form as: Cell Rep. 2016 Nov 15;17(8):2004–2014. doi: 10.1016/j.celrep.2016.10.073

Figure 7. RNA Editing Promotes the Binding of CAPS1 to the Activated Form of Syntaxin-1A.

Figure 7

(A) The amount of endogenous syntaxin-1A in mouse brain homogenate that bound to either unedited or edited Halo-human CAPS1 in pull-down assays was analyzed by western blotting. The expression level of CAPS1 is shown as a reference.

(B) The relative amount of endogenous syntaxin-1A bound to CAPS1 was compared between the unedited and edited isoforms. Values are displayed as the mean ± SEM (n = 3; Mann-Whitney test, *p < 0.05).

(C) The amount of syntaxin-1A in either the closed (CI) or open (Op) conformation that bound to either unedited or edited Halo-human CAPS1 in pull-down (IP) assays was analyzed by western blotting. The expression of CAPS1 is shown as a reference.

(D) The relative amount of syntaxin-1A in either the closed (CI) or open (Op) conformation bound to either unedited or edited CAPS1 was compared. All values are displayed as the mean ± SEM (n = 6; one-way ANOVA with Wilcoxon test, *p < 0.05). The mean value of the relative amount of closed conformation syntaxin-1A bound to unedited CAPS1 was set as one.