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. 2016 Dec 22;17(12):3319–3332. doi: 10.1016/j.celrep.2016.11.061

Figure 3.

Figure 3

JM-Y687 Promotes RET Catalytic Activity and Rescues a Catalytically Deficient RET Activation-Loop Mutant Lacking Tyrosine

(A) WB analysis of purified recombinant RET JM661-KD, JM678-KD, and JM-698-KD (2.5 μM) WT and Y687F mutants stimulated with ATP (5 mM) and MgCl2 (10 mM) for 0–80 min using the indicated antibodies.

(B) WB analysis of purified recombinant RET JM661-KD (2.5 μM) WT and indicated Y/F mutants (Y900F, Y905F, Y900/905F, Y981F) with ATP (5 mM) and MgCl2 (10 mM) for 0–80 min using the indicated antibodies.

(C) WB analysis of purified recombinant RET JM661-KD and RET KD (2.5 μM) WT, Y900/905F (YY/FF), and Y687/900/905F (YYY/FFF) mutants as indicated stimulated with ATP (5 mM) and MgCl2 (10 mM) for 0–90 min using the indicated antibodies. Total amount of protein was assessed by Coomassie blue staining.