Figure 3.

Alternative 3′ End Processing of SLM2 mRNA Is Modulated in Response to Increasing SLM2 Protein Concentrations Leading to mRNA Instability

(A) Distribution of potential binding sites for SLM2 protein (purple arrowheads) in the downstream portion of the SLM2 gene of human, mouse, and chicken. Exons are shown as boxes. The canonical mRNA 3′ end is shown in red with its normal poly(A), and the alternative 3′ end (AS) in blue.

(B) Western blot showing tetracycline-induction of SLM2-flag tagged protein within a stable HEK293 cell line as compared to a control cell line (made with empty pCDNA3 vector), detected using α-FLAG antibodies (α-actin used as a loading control).

(C) Agarose gel showing induction of SLM2 alternative end 2 is induced by tetracycline treatment and stabilized by cycloheximide treatment.

See also Figure S2.