Figure 5.

Splicing Targets Controlled by SLM2 within the CA1–CA3 Regions Have Flanking Intron Sequences Containing UWAA Motifs

(A) Distribution of UWAA sequences flanking exons that change splicing patterns within the Slm2 KO mouse. A 1-kb window of the mouse genome is shown, with the SLM2 regulated exon in red, surrounded by flanking intron sequence. For each genomic region, the distribution of UAAA and UUAA repeats are shown in purple.

(B) Potential Sam68 and SLM2 target sequences were cloned into pBluescript and transcribed into radiolabelled RNA probes. RNA-protein interactions were confirmed by gel shifts (right panels) with RNA sequences shown at the left (UWAA binding sites are highlighted in red for UAAA, and yellow for UUAA. Note a number of UAAA and UUAA sites are overlapping).

See also Figure S3.