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. Author manuscript; available in PMC: 2017 Mar 6.
Published in final edited form as: Mucosal Immunol. 2016 Jun 29;10(2):434–445. doi: 10.1038/mi.2016.55

Figure 1. NLRP6 is induced in lamina propria Ly6Chi monocytes during DSS induced inflammation, and is important for reducing susceptibility to colitis.

Figure 1

(a) NLRP6 levels were measured in BM, LP, IECs and IELs of WT mice at day 0 and day 10 of AOM/DSS by qPCR. (b) NLRP6 expression was measured in CD3+B220− CD11b− T cells, CD3− B220+CD11b− B cells, CD3− CD11b+Ly6ChiLy6G− monocytes and CD3− CD11b+Ly6CintLy6G+ neutrophils within the BM and LP. Data are representative of three independent experiments; n=11 for day 0, n=10 for day 10. *, ** - p<0.05, p<0.001, respectively, as compared to day 0 time point of the same genotype. (c) Representative plots of Ly6C versus Ly6G staining of CD11b+ BM cells (top). Kaplan–Meyer survival curves of mice treated with 7 days of 3.5% DSS (bottom). (d) Percent weight change with 3.5% DSS administration. Data are representative of two independent experiments; n=15, n=24, n=14 for WT, Nlrp6−/− and Nlrp6−/− + WT Ly6Chi monocytes groups respectively. *, ** - p<0.05, p<0.001, respectively, as compared to Nlrp6−/−.