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. Author manuscript; available in PMC: 2017 Mar 6.
Published in final edited form as: Mucosal Immunol. 2016 Jun 29;10(2):434–445. doi: 10.1038/mi.2016.55

Figure 4. NLRP6 deficient Ly6Chi monocytes have reduced ROS and TNFα production during the acute inflammatory response to DSS.

Figure 4

(a) Ly6Chi monocytes were sorted from WT or Nlrp6−/− mice and incubated with GFP-labeled E.coli at MOI 0, 5, 25 and 60. Phagocytosis was measured by percent GFP+ Ly6Chi monocytes. Data are representative of two independent experiments, n=6. ROS production (MFI) as measured by CellROX deep red in BM- (b) or LP-derived (c) Ly6Chi monocytes at the indicated time points. Ly6Chi monocytes from WT or Nlrp6−/− mice were sorted from the LP after 5 days of 2% DSS (day 10, AOM/DSS model) or 6 days after completion of DSS (day 16, AOM/DSS model) and cytokine levels were measured by ELISA (d) and by qPCR (e). Data are representative of at least three independent experiments, mean ± SEM; n=14/genotype, (day 10); n=12/genotype (day 0); n=8/genotype (day 16). * - p<0.05, as compared to WT.