Figure 6. Inhibition of α₅β₁ protects against airway hyperresponsiveness and enhances the effect of bronchodilators.

(A) Pulmonary resistance measurements in WT C57Bl/6 mice following immunization and i.n. challenge with OVA, with i.n. administration of ATN-161 (12.5 mg/kg) or vehicle (5% DMSO, 0.9% saline) 1 hour prior to measurements. n = 8 animals per group. (B) Tension of mouse tracheal rings measured after 12 hours in DMEM with IL-13 (100 ng/ml) and incubation for 15 minutes with Mch (10^–6 M) in response to a range of concentrations of the β-adrenergic agonist isoproterenol in the presence of ATN-161 (100 μg/ml) or vehicle. n = 5–8 rings per group. Data represent the mean ± SEM. **P < 0.01 and ***P < 0.001; repeated measures of variance.