Figure 4. Irf8^–/– mice have a block in terminal NK cell maturation.

Spleen and blood from Irf8^–/–, Irf8^+/– littermates, and Irf8^+/+ controls were analyzed by flow cytometry for markers of NK cell differentiation. (A) Representative FACS of spleen (top) and blood (bottom) from the same mouse. Cells were gated on single cells, then NK1.1⁺CD3⁻. (B) Pooled spleen and blood from 4–6 mice per condition are shown. Data shown are mean ± SD. *P < 0.05 by Student’s unpaired t test.