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. 2016 Sep 15;595(1):141–164. doi: 10.1113/JP272950

Figure 8. Baclofen improves rotarod motor performance in SCA1‐Tg mice and the improvement corresponds to restoration of mGluR signalling.

Figure 8

A, motor performance examined by an accelerating rotarod test. Baclofen (5 nm) or PBS was applied to the cerebellum of 12‐week‐old SCA1‐Tg mice 3 h before the rotarod test on day 0 (inj.). aThe sample size is different on days 14 and 21 (27 mice). B and C, transient recovery of mGluR‐mediated slow EPSCs (B) and SSE (C) in cerebellar PCs obtained from the baclofen‐treated SCA1‐Tg mice. This recovery effect lasted until day 7 (red) but vanished at day 21 (grey) after a single application of baclofen (5 nm) to the cerebellum of SCA1‐Tg mice. Upper panel in C shows representative basal EPSCs (broken lines, the last EPSCs just before SSE induction) and the following second EPSCs after SSE induction (continuous lines). D, rescue of LTD in PCs from the baclofen‐treated SCA1‐Tg mice at day 7 after the single baclofen infusion to 12‐week‐old SCA1‐Tg mice (red), in comparison with the PBS‐treated SCA1‐Tg mice at day 7. Upper panel shows fast EPSC traces representative of the baseline responses (broken lines, the last EPSC before LTD induction) and the EPSC 30 min after the conjunctive stimulation for LTD. In C and D, induction stimuli for SSE and LTD were applied at time 0. * P < 0.05; ** P < 0.005. [Colour figure can be viewed at wileyonlinelibrary.com]