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. 2016 Dec 1;6(12):2816–2830.

Figure 7.

Figure 7

Over-expression of YAP attenuates the antitumor activity of verteporfin. (A) YAP was critical in verteporfin-induced apoptosis in UM cells. After treated with control or 5 μM verteporfin for 24 hours, Omm 2.3 cells stably expressing HA-YAP or empty vector underwent trypan blue exclusion assay and Western blotting analysis. (B, C) YAP promoted migration of UM cells. After treated with or without 0.5 μM verteporfin, HA-YAP-overexpressing Omm 2.3 cells underwent migration (B) and invasion (C) assay. (D) Overexpression of YAP rescued the verteporfin-induced decrease in capacity of melanosphere formation. Omm 2.3 cells stably expressing HA-YAP or empty vector were exposed to 0.25 μM verteporfin, and inoculated for melanosphere assay in drug-free DMEM/F-12 medium for 14 days. Columns, mean; bars, SEM. *, P<0.05; **, P<0.01; ***, P<0.001. One-way ANOVA with post hoc intergroup comparison by the Tukey test.