Table 4.

Detection of apoptotic cells

A549	Region	Events	% Gated	% Total	X Mean	X Geo Mean	Y Mean	Y Geo Mean
Ctr	R1	9237	100.00	92.37	8.42	7.27	438.56	417.68
	R2	17	0.18	0.17	154.21	120.98	618.82	588.02
CDDP 70% IC50	R1	5951	100.00	59.51	9.05	6.27	463.55	443.41
	R2	26	0.44	0.26	156.55	64.74	418.65	404.21
VPA 70% IC50	R1	9626	100.00	96.26	15.00	8.78	469.60	428.63
	R2	404	4.20	4.04	141.39	101.77	453.67	409.01
VPA+CDDP 70% IC50	R1	9553	100.00	95.53	29.80	12.92	591.59	557.49
	R2	1260	13.19	12.60	155.83	127.96	532.78	502.44
SAHA 70% IC50	R1	8860	100.00	88.60	9.50	7.36	311.01	278.37
	R2	76	0.86	0.76	88.89	64.58	336.67	300.38
SAHA+CDDP 70% IC50	R1	7551	100.00	75.51	15.15	9.80	531.33	504.17
	R2	191	2.53	1.91	163.22	120.11	492.93	471.69
CDDP 120% IC50	R1	9723	100.00	97.23	13.80	10.38	557.91	537.33
	R2	335	3.45	3.35	100.21	87.73	445.07	420.25
VPA 120% IC50	R1	9614	100.00	96.14	10.66	7.62	399.43	374.29
	R2	119	1.24	1.19	191.94	153.40	363.56	334.57
VPA+CDDP 120% IC50	R1	9754	100.00	97.54	20.06	8.60	440.97	421.18
	R2	2332	23.91	23.32	66.30	59.62	461.03	442.99
SAHA 120% IC50	R1	9585	100.00	95.85	9.24	7.97	484.04	458.75
	R2	22	0.23	0.22	146.17	117.48	464.23	427.33
SAHA+CDDP 120% IC50	R1	9678	100.00	96.78	14.61	9.86	433.49	413.62
	R2	1173	12.12	11.73	56.64	49.96	391.34	374.33

A549 lung cancer cell line was incubated with active agents alone or in mixture of them with doses 1.4 = 70% IC50 and 2.4 = 120% IC50 for 48 h. Data was analyzed by flow cytometry.