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. 2016 Dec 1;6(12):2846–2858.

Figure 4.

Figure 4

Antigen-binding ability and tumor growth inhibition of cdGIGPQc-ScFv in vitro. A: The antigen-specific binding of ScFv and cd-ScFv was analyzed via Western blot analysis. After human ITG αvβ3 was imprinted, ScFv and cd-ScFv detected a protein band similar to that by positive control mAb, as primay atibodies. B, C: Human lung adenocarcinoma cell A549 and human lung adenosquamous carcinoma cell L78 were incubated with indicated concentrations of cdGIGPQc-ScFv, cNAQAEQc-ScFv or control ScFv for 24 h and the cell proliferation was checked by CCK8 assay. cdGIGPQc-ScFv showed a superior antitumor effect than ScFv and cNAQAEQc-ScFv. Data were from one of independent triplicate experiments.