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. 2016 Dec 1;6(12):2901–2909.

Figure 2.

Figure 2

Delineation of the domains mediating the mutual interactions between USP11 and VGLL4. A. Schematic representation of VGLL4 truncation showed in the study. NLS, nuclear localization signal. NCS, novel conserved sequence. The ability of each VGLL4 deletion mutants to bind to USP11 is indicated (+: binding, -: no binding). B. 293T cells were co-transfected with Myc-USP11 and the indicated FLAG-VGLL4 or deletion mutants, cell lysates were prepared and mmunoprecipitated with anti-FLAG antibody, followed by WB analyses with indicated antibodies. C. Schematic presentation of USP11 domains and deletion mutants. The ability of each USP11 deletion mutants to bind to VGLL4 is indicated. D. 293T cells were co-transfected with Myc-VGLL4 and the indicated FLAG-USP11 or deletion mutants, cell lysates were prepared and immunoprecipitated with anti-FLAG antibody, followed by WB analyses with indicated antibodies.