Figure 2.

Physcion induces apoptosis in caspase-dependent manner in HCC cells. HCC cells were treated with physcion for 48 hours before assay were performed. A. Physcion treatment correlates with dose-dependent increase in caspase-3 activity. B. Cleaved caspase-3 level is elevated in cells treated by physcion. C. AIF and Endo G are not involved in the physcion-mediated apoptosis. D. Caspase inhibitor z-VAD-FMK significantly abrogates the apoptosis-inducing effect of physcion in HCC cells. **P<0.01.