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. 2016 Dec 1;9(12):1483–1495. doi: 10.1242/dmm.023366

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — HDAC9 regulates cell cycle progression. (A) Immunoblot analysis of HDAC9 expression in untreated wild-type Raji B-cell non-Hodgkin lymphoma (B-NHL) cells (control, lane 1), and the mutants GFP ZFN-derived control (lane 2) and HDAC9 ZFN-derived cells prior to single cell sorting (lane 3). Immunoblot analysis of GAPDH expression was used as loading control. (B) Cell growth curve of wild-type Raji control and representative ZFN-generated HDAC9 mutant clones 3B2 and 3G9 obtained by single-cell sorting. (C) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of proliferation of HDAC9 ZFN-derived clones. Shown are values for HDAC9 ZFN-derived mutant clones 3B2 and 3G9, a pool of unsorted HDAC9 ZFN mutants, and wild-type cells and untreated Raji cells. (D) Effects of HDAC9 depletion on cell cycle progression are depicted in histogram plots generated by propidium-iodide uptake.