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. 2016 Dec 1;9(12):1483–1495. doi: 10.1242/dmm.023366

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — Eµ-HDAC9 tumors display deregulated acetylation of BCL6 and p53. (A) IHC triple-immunostaining using the ABC-TSA method in mouse spleens from Eμ-HDAC9 and wild-type tumors, showing HDAC9 (red) expression in conjunction with levels of acetylated (Ac)-BCL6 (green). (B) Immunoblot analysis of Ac-p53 in Eµ-HDAC9 and wild-type spleen. GAPDH was used as a loading control. (C) ABC-TSA immunofluorescence analysis of Ac-p53 (green) and HDAC9 (red) in spleen in Eμ-HDAC9 and wild-type controls.