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. 2016 Oct 28;5(12):1784–1789. doi: 10.1242/bio.019620

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Relative expression of supporting pathways for beta-lactam biosynthesis in F. candida after heat shock. Gene expressions were determined from qPCR-derived cycle threshold values for cDNA amplification relative to two reference genes. Points depict observed means for each sampling time (n=4) and error bars depict the standard error of the means as a function of time. Blue bullets, samples that received heat shock; red squares, controls. The grey column represents the time of the heat shock. Asterisks depict significance level, (*P<0.05; **P<0.01; ***P<0.001) from the two-way ANOVA analysis in R 3.2.2.