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. 2016 Nov 23;14(6):371–378. doi: 10.1016/j.gpb.2016.06.002

Figure 2.

Figure 2

Gel electrophoresis of human fecal metagenomic DNA isolated with different methods Metagenomic DNA isolated from fresh human feces (A) or one-month-old human feces stored at −86 °C (B) using different methods was separated on 0.8% agarose gel. Phage λ DNA EcoR1/HindIII digest was used as DNA marker. Method A, Power Fecal® DNA Isolation Kit from MO BIO Laboratories; method B, Extract Master™ Fecal DNA Extraction Kit from Epicentre; method C, Favor Prep™ Stool DNA isolation Kit from Favorgen Biotech; method D, QIAamp DNA Stool Kit from QIAGEN; current method, method presented in the current study. Metagenomic DNA was extracted and eluted per manufacturers’ instruction. The volume of DNA elutions was normalized according to the starting amount of fecal samples and then equal volume of DNA was used for uniform sample loading to perform comparison among different methods.

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