Pairwise comparison of the fold, active site
clefts, and key catalytic
residues of SsGH134 and representative glycoside
hydrolases with lysozyme folds. SsGH134 E45Q (PDB 5JUG, light green) with
M5 bound (blue) overlaid with (a) retaining GH22 family HEWL (PDB 2WAR, yellow) with chitopentaose
bound (plum), (b) inverting GH19 family chitinase A (PDB 3WH1, pink) with chitobiose
and chitotriose bound (magenta), (c) inverting GH124 family cellulose CtCel124 (PDB 2XQO, lavender) with two molecules of cellotriose bound
(chocolate), (d) inverting GH23 G-type lysozyme (PDB 3GXR, dark green) with
chitobiose and chitotriose bound (brown). (e) Close up overlay of
the catalytic residues of SsGH134 (light green),
HEWL (yellow), chitinase A (pink), CtCel124 (lavender),
and G-type lysozyme (dark green).