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. 2017 Mar 23;5:2825. Originally published 2016 Dec 6. [Version 2] doi: 10.12688/f1000research.10336.2

Figure 2. Comparison of two commercially available rabbit polyclonal anti-SK2 antibodies for immunoprecipitation of SK2 from HEK293 cell lysates.

Figure 2.

SK2 was immunoprecipitated from HEK293 cell lysate using either ( A) Proteintech rabbit anti-SK2 antibody or ( B) ECM Biosciences rabbit anti-SK2 antibody. Normal rabbit IgG antibody was used as an isotype control. Immunoprecipitates (and 40 µg lysate input) were subjected to immunoblot analyses and probed with ( A) Proteintech rabbit anti-SK2 antibody or ( B) ECM Biosciences rabbit anti-SK2 antibody. Membranes were imaged using a 4 min exposure. Images are representative of three independent experiments for each antibody. ( C) SK2 was immunoprecipitated from HEK293 cell lysates (of equal protein) that had been treated with scrambled control siRNA (si-Neg) or SK2 siRNA (si-SK2), using ECM Biosciences rabbit anti-SK2 antibody. Immunoprecipitates were subjected to immunoblot analyses and probed with ECM Biosciences rabbit anti-SK2 antibody. Membrane was imaged using a 4 min exposure. Image is representative of three independent experiments. IgG h/c = IgG heavy chain.