Table 3. Reagents used for immunoprecipitation.
| Process | Reagent | Manufacturer | Catalogue
number |
Concentration |
|---|---|---|---|---|
| Magnetic Labelling (µMacs) | Protein A µBeads
Protein G µBeads |
Miltenyi Biotech | 130-071-001
130-071-101 |
Proprietary |
| Washing Columns | EB -DTT (described in methods)
Low Salt Wash Buffer: Tris/HCl pH 7.5 |
Invitrogen |
15504-020 |
Various
20 mM |
| 1× Laemmli Protein Loading
Buffer |
Tris/HCl pH 8.0
EDTA Dithiothreitol (DTT) SDS Glycerol Bromophenol blue |
Invitrogen
Merck Millipore Sigma Aldrich Sigma Aldrich Chem-Supply Sigma Aldrich |
15504-020
108421 D0632 75746 GA010 B5525 |
10 mM
1 mM 20 mM 1% w/v 10% v/v 0.02% w/v |