Table 3. Top 50 genes that at 24 h after SCI were either up-regulated or down-regulated in response to PcTx1 treatment.
Data are shown for the injury centre segment and the immediately adjacent rostral and caudal segments for animals with SIS 2.5 injuries (a) or SIS 2.75 injuries (b). Fold changes (FC) were calculated from FPKM values (see Methods).
| SIS 2.75 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Rostral | Injury Centre | Caudal | |||||||||
| Up-regulated | Down-regulated | Up-regulated | Down-regulated | Up-regulated | Down-regulated | ||||||
| Gene | FC | Gene | FC | Gene | FC | Gene | FC | Gene | FC | Gene | FC |
| Cxcl2 | 20.2 | Micb | -18.5 | Slc17a7 | 41.1 | Myh1 | -29.8 | Scn10a | 74 | Mmp10 | -39.7 |
| Dnah8 | 18.4 | Slc19a3 | -12 | Kcnh7 | 27.7 | Actn3 | -29.3 | Ckm | 73 | Serpinb2 | -32.2 |
| Rn60_X_0663.2 | 16.2 | Slco1c1 | -11.6 | Ksr2 | 26.8 | Ereg | -27.1 | Mylpf | 42.3 | LOC100911361 | -32.2 |
| Gabrp | 16.2 | Gm26965 | -11.1 | Skor1 | 26.8 | Hp | -26 | Avil | 28.6 | Acap1 | -21.8 |
| Il31ra | 11.9 | Npas1 | -10.2 | Frmpd3 | 25.8 | Treml2 | -21.7 | Car3 | 22.9 | Fst | -18.9 |
| LOC100361444 | 9.7 | Slc44a3 | -10.2 | Lbx1 | 22.1 | Tnnt3 | -19.5 | Tnnt1 | 16.9 | Siglech | -18 |
| Stfa2l3 | 8.6 | Siglec8 | -10.2 | Opn4 | 17.5 | Arl5c | -18.4 | Scn11a | 16.9 | Ccl12 | -17.4 |
| Il2ra | 8.6 | Kcns2 | -9.7 | Grin2a | 16.8 | Ccdc69 | -16.3 | Myh1 | 16.1 | RGD1309870 | -16.1 |
| Cxcl3 | 8.3 | Cxcl17 | -9.3 | RGD1308544 | 16.6 | Acap1 | -16.3 | Actn3 | 13.7 | AABR07030791.1 | -15.1 |
| Il1rl2 | 8.1 | Kcnh6 | -8.8 | Rasal1 | 14.8 | Itga2 | -16.3 | Gabrq | 13.7 | Il11 | -13.6 |
| Tfap2a | 7.9 | Akr1c19 | -7.4 | Frem3 | 14.8 | Tbx15 | -16.3 | Kcnh8 | 12.7 | Rpl17 | -13.4 |
| Twist2 | 7.9 | Tph2 | -7.4 | Lypd6b | 14.1 | Il4i1 | -16.3 | Sprn | 10.6 | Fcrla | -13.2 |
| Arl5c | 7.6 | Tctex1d1 | -7.4 | AABR07065531.5 | 13.8 | RNaseP_nuc | -15.6 | Acta1 | 10.2 | Cxcl6 | -12.8 |
| AABR07053509.2 | 7.6 | Ccdc13 | -7.1 | Slc26a8 | 13.8 | Il11 | -14.6 | Tyrp1 | 9.5 | Slc39a12 | -11.2 |
| Clca4l | 7.5 | Rpl30 | -7.1 | Nmu | 13.8 | AABR07021998.1 | -14.1 | AABR07070246.1 | 9 | Trib3 | -11 |
| Fam111a | 7.4 | Fam183b | -6.9 | Lgr5 | 13.8 | Irg1 | -13.5 | RGD1560672 | 9 | Gabrp | -10.4 |
| Cyp4f18 | 7 | Hpx | -6.8 | Slc27a2 | 13.5 | RNase_MRP | -13.1 | Myl1 | 8.7 | LOC100909752 | -10.4 |
| Dusp5 | 7 | AABR07000733.1 | -6.6 | Hapln1 | 13.5 | Irf4 | -13 | Siglec8 | 8.6 | Gdf15 | -10.4 |
| Lrrc15 | 6.7 | RGD1306233 | -6.5 | RGD1560672 | 12.9 | Cytl1 | -13 | Tusc5 | 8.3 | Dnah8 | -9.5 |
| Nppb | 6.5 | Hapln1 | -5.8 | Crhbp | 12.9 | Hcst | -12.6 | Isl2 | 7.9 | Cxcl3 | -9.2 |
| Gdnf | 6.5 | Kcng1 | -5.7 | Shisa6 | 12.3 | Cxcl2 | -12 | Abca6 | 7.4 | Serpine1 | -8.6 |
| Cyp27b1 | 6.5 | Gm1043 | -5.6 | AABR07045621.1 | 12 | LOC684828 | -11.9 | LOC100359656 | 6.9 | Hsf2bp | -8.5 |
| Oas3 | 6.5 | Plin4 | -5.6 | Mfi2 | 12 | Gdnf | -11.6 | Zap70 | 6.7 | Fam83g | -8.5 |
| Ucn2 | 6.4 | Chrm4 | -5.2 | AABR07070246.1 | 12 | Tmprss11f | -11.6 | Ifit1 | 6.3 | Sp5 | -8.5 |
| AC091336.1 | 5.9 | Creg2 | -5.2 | Cfap126 | 12 | Myct1 | -10.8 | Slc26a3 | 6.3 | AABR07044837.2 | -7.9 |
| Snai2 | 5.9 | Chrm4 | -5.2 | Gpr61 | 11.8 | Pf4 | -10.8 | Gprin3 | 5.8 | Ccl17 | -5.7 |
| Igj | 5.8 | Tecta | -5.2 | Myadml2 | 11.5 | RGD1565355 | -10.7 | Zfp773 | 5.3 | Oip5 | -5.7 |
| Hsh2d | 5.8 | AABR07034833.1 | -5.1 | Gbx1 | 11.1 | Nppb | -10.3 | Mfi2 | 5.3 | Irg1 | -5.7 |
| Trh | 5.7 | Dnah5 | -5.1 | Fbxl18 | 11.1 | Dusp2 | -10 | Esrrb | 5 | Ppp1r32 | -5.7 |
| Il11 | 5.4 | Draxin | -5.1 | Fstl4 | 11.1 | Slpi | -9.9 | Clic6 | 5 | Cd7 | -5.7 |
| Slamf6 | 5.4 | Gpr101 | -5 | Htr4 | 11.1 | Selp | -9.8 | Ctxn3 | 5 | Ccl2 | -5.8 |
| Msc | 5.4 | Enkur | -4.9 | Slc19a3 | 11.1 | Krt12 | -9.8 | Dpf3 | 4.9 | Mis18a | -5.9 |
| Echdc3 | 5.4 | RGD1304810 | -4.9 | Ky | 11.1 | Galnt5 | -9.8 | Icam5 | 4.8 | Dusp2 | -6 |
| Rn60_7_1164.1 | 5.1 | Myl1 | -4.6 | Pip5kl1 | 11.1 | Trem3 | -9.5 | Ibsp | 4.8 | Fam111a | -6 |
| Lif | 5.1 | Kcp | -4.6 | Pax2 | 10.8 | Fcnb | -9.5 | Lypd8 | 4.8 | Ucn2 | -6.2 |
| Osm | 5.1 | Rn60_X_0744.3 | -4.6 | Vsx2 | 10.8 | RGD1305807 | -9.2 | Nox4 | 4.8 | Phldb3 | -6.2 |
| Osr2 | 5 | Pgr | -4.6 | Gjd2 | 10.7 | Col28a1 | -9.2 | Itih2 | 4.8 | Btc | -6.2 |
| Nox4 | 5 | Dok7 | -4.6 | Dok7 | 10.6 | Retnlg | -8.9 | LOC299282 | 4.7 | Pfkfb4 | -6.3 |
| Irg1 | 5 | Opn4 | -4.6 | Efna5 | 10.6 | Osm | -8.8 | 4932411E22Rik | 4.6 | Mar-01 | -6.6 |
| Rhbg | 5 | Mybpc1 | -4.6 | Tctex1d1 | 10.2 | Trh | -8.8 | P2rx3 | 4.6 | Itga2 | -6.6 |
| Il1r2 | 4.9 | Chodl | -4.5 | Ppp1r3g | 10.2 | Dsc2 | -8.7 | Slc22a2 | 4.3 | Rpl12 | -6.6 |
| AABR07001634.1 | 4.9 | Cdh12 | -4.5 | Cxcl17 | 10.2 | Cyp4f18 | -8.7 | Mybpc1 | 4.2 | Gdnf | -6.6 |
| Mmp8 | 4.8 | RGD1308544 | -4.4 | Wnt3 | 10.2 | Il17re | -8.7 | Gabre | 4.2 | Lif | -6.6 |
| LOC24906 | 4.8 | Gdpd2 | -4.4 | Neurod6 | 10.2 | Il1rl2 | -8.7 | RNase_MRP | 4.2 | Clca4l | -6.8 |
| Myod1 | 4.6 | Akr1b7 | -4.4 | Hecw1 | 10 | Fam60a | -8.7 | B3gntl1 | 4.2 | Fabp4 | -6.9 |
| Rufy4 | 4.5 | LOC498435 | -4.3 | Cpne9 | 10 | Gfra3 | -8.7 | Btbd17 | 4.2 | Eif5b | -7 |
| Myo18b | 4.5 | Perp | -4.3 | RGD1564053 | 9.7 | Ucn2 | -8.5 | Glb1l2 | 4.2 | Mmp3 | -7 |
| Pglyrp1 | 4.5 | Spag1 | -4.3 | Col25a1 | 9.7 | Fabp4 | -8.5 | Tnfsf10 | 4.1 | Ereg | -7.6 |
| Fabp4 | 4.5 | RGD1564053 | -4.2 | Cntn6 | 9.6 | Ccr7 | -8.3 | AC141169.2 | 4.1 | Lamb3 | -7.6 |
| AC115145.1 | 4.4 | Cntnap5a | -4.2 | Abcc8 | 9.6 | Kazald1 | -8.3 | Oprk1 | 4.1 | Ccl7 | -7.7 |