Table 1.
Calculated and Experimental Sizes of Different Molecular Weights of HA
| MW (kDa) |
calcd radius of gyration (Rg) (nm) |
calcd hydrodynamic radius (Rh) (nm) |
DLS measurements (nm) |
size based on SEC data (kDa)b |
SEC HA-tyrosine (kDa)b |
calcd viscosity (dL/g) |
|---|---|---|---|---|---|---|
| 7 | 7.1 | 3.0 | 6.1 ± 0.2 | 14 ± 3 | 13 ± 2 | 14.8 |
| 30a | 16.3 | 7.4 | 12.2 ± 1.3 | 68 ± 7 | 59 ± 4 | 68.9 |
| 67 | 25.8 | 12.3 | 22.1 ± 1.2 | 155 ± 14 | 127 ± 9 | 161.0 |
| 215 | 50.2 | 25.6 | 33.3 ± 0.2 | 557 ± 28 | 435 ± 18 | 477.3 |
| 741 | 101.6 | 53.9 | 55.0 ± 0.7 | 1646 ± 51 | 451 ± 25 | 1250.0 |
a
Calculations based on an estimated molecular weight of 30 kDa HA. Lots were 29 kDa and 31 kDa. DLS and SEC data were performed for the 29 kDa HA lot.
b
Standard deviations were based on running the same samples for three different runs.