Table 4.

Amino acid position differences between Global and High Local reassortant lineages

		Global reassortant lineage			High Local reassortant lineage
					N America		SC America	Asia		Australia	M Easta
	Segment/Position	X (Red)	XII (Dark Red)	III (Light Blue)	XI (Maroon)	XIV (Purple)	VII (Green)	IV (BlueD)	XIII (Orange)	VI (GreenL)	II (Dark Blue)a
Shannon entropy differences	NA/151	D/N/G	D/N/G	D/N	D	D/ G	D	D	D	D	D/N
	HA/215	A/S	A/S	S	S	S	S	S	S	S	S
Amino acid differences Global vs. Local	PB2/81	I	I	I	M	M	M	M	M	M	M
	PB2/255	I	I	I	V	V	V	V	V	V	V
	PB2/392	D	D	D	E	E	E	E	E	E	E
Amino acid differences Local	PB2/221	S	S	A	S	S	A	A	A	A	A
	PB2/293	K	K	R	K	K	R	R	R	R	R
	PB2/353	K	K	R	K	K	R	R	R	R	R
	PB2/560	I/V	I/V	I/V	I	I	V	V	V	V	V

^aAlthough assigned High Local by the criteria, it cannot with certainty be regarded as such due to low regional and temporal sampling. It was included for exploratory purposes only

D/N Position was not conserved in this reassortant lineage, and the Shannon entropy of the Global reassortants was not significantly higher in any of the pairwise comparisons

D/ _G Only one of the reassortant genomes had a differing amino acid in this position. Shannon entropy of the Global reassortants was not significantly higher in any of the pairwise comparisons

D Position was conserved in these reassortant lineages, but the Shannon entropy of the Global reassortants was not significantly higher in any of the pairwise comparisons