Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 1;129(23):4341–4353. doi: 10.1242/jcs.193904

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 2. — Rab40b is required for invadopodia extension and maturation. (A–D) MDA-MB-231 cells, wild-type (A,B) or stably expressing Rab40b shRNA (KD1 or KD2; C,D) were embedded in Matrigel+collagen I mixture and grown for 4 days. The spheroids were then fixed and stained with anti-tubulin (green) and phalloidin (red) antibodies to mark mature invadopodia. In B, wild-type spheroids were treated with 50 µM of MMP2 and MMP9 inhibitor (Inh.) SB3CT for the last 48 h of 4 day growth. The arrows in A and D indicate invadopodia. (E,F) Invadopodia length (F) and number of spheroids with invadopdodia (E) were analyzed for each set of cells. Data shown are the mean±s.d. from three independent experiments. n is the total number of spheroids analyzed. P-values given are compared to control and calculated with a two-tailed Student's t-test.