Fig. 3.

SIRT2 deacetylates the regulator of nuclear envelope reassembly ANKLE2. (A) Western blots of anti-HA co-immunoprecipitation (IP) from asynchronous and mitotic HEK293T cells overexpressing HA–SIRT2 and ANKLE2–GFP. HA–PARP3 was used as a negative control. (B) In vitro acetylation of recombinant ANKLE2 with 0.15 μg of p300, PCAF and CBP acetyltransferases. hMOF did not acetylate ANKLE2. 0.015, 0.15 or 1.5 μg of ANKLE2 was present in the loaded sample. (C) In vitro deacetylation of ANKLE2 by SIRT2. 1.5 μg of ANKLE2, 0.15 μg of acetyltransferase and 0.15/1.5 μg of SIRT2 or SIRT2 H150Y were present in the loaded samples.