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. 2016 Dec 15;129(24):4576–4591. doi: 10.1242/jcs.194035

Fig. 3.

Fig. 3.

iPSC colonies formed from Aplf-kd cells express pluripotency genes. (A) C21 and C23 iPSC clones at passage 26. Scale bar: 100 µm. (B) iPSCs from colonies C21 and C23 (generated from Aplf-kd MEFs), and C3 (generated from control plko.1 MEFs) were analyzed for the expression of the pluripotency proteins OCT3/4 and NANOG by performing immunofluorescence analysis. E14 ESCs were used as control. (C) qRT-PCR analysis of different pluripotency genes on samples generated from different passages (p-) of E14 ESCs and of iPSCs from C21, C23 and C3. Error bars represent s.e.m. for three independent experiments.