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. 2016 Dec 15;129(24):4449–4454. doi: 10.1242/jcs.196337

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Dictyostelium deficient in the DNA repair nuclease Xpf present a growth defect on Klebsiella aerogenes lawns but not in axenic medium. (A) Wild-type (Ax2) and xpf-deficient (xpf⁻) Dictyostelium were plated on agar plates coated with K. aerogenes (K.a.); single clones of Dictyostelium grow out as punched colonies. (B) Quantification of clonal growth of the two strains on K. aerogenes plates, scored as plaque diameter at day 5 after growing at 22°C (n=55 and n=53 for Ax2 and xpf⁻, respectively). (C) Colony-forming efficiency of Ax2 and xpf⁻ on K. aerogenes plates (n=20). (D) Growth curves for Ax2 and xpf⁻ in axenic medium. (E) Doubling times calculated from the plot in D (n=3, mean±s.e.m.). (F) Colony-forming efficiency in axenic medium (n=20). ****P<0.0001; n.s., not significant (t-test).