Fig. 1.

Multilineage differentiation and genetic variability of conventional hiPSCs derived from unique donors via different reprogramming methods. (A) Hematopoietic differentiation [% day 14 human embryoid body (hEB) cells] of independent donor-derived hPSCs (see Table S2). hESC lines: H9, H7, H1, ES03 (gold). Fibro-iPSCs: IMR4, HUF3, HUF5, 7ta, WT2 (green). 7F-E and 4F-E hematopoietic-iPSCs: lines generated without stromal activation (sa) were mononuclear CB-7F-E (iCB 2.5, iCB8, iCB9; purple) and MP-CB-4F-E (E17C1, E20C2, E32C7; purple) and lines generated with sa were saMPCB-4FE (E5C3, E12C5, E17C6; red). Included are MP-PB-4FE lines from the same donor (ZPB) that were derived with sa (E29C1, E29C4, E29C6; red) and without sa (E29C10, E29C11, E29C12; purple). Four 7F-E CB-derived sa-MP-iPSC lines were all from the same donor (D003) generated with sa (6.2, 6.13, 19.11) (Fig. S2). *P<0.05, **P<0.005 (one-way ANOVA) for averaged groups relative to the hESC group. (B) Osteogenic differentiation (quantitative Alizarin Red dye), and (C) endodermal differentiations of individual lines in each hPSC class from the same unique donors as above. Error bars indicate s.e.m. (D) Number of genes differentially expressed between hiPSCs and hESCs (# hiPSC ≠ hESC) (ANOVA, P<0.05; fold change >1.5×). (E) Dendrogram of hPSCs from above clustered on lineage-primed gene targets of the PRC2 complex (Table S1). (F) PCA of ESC and core module genes (Table S1) showing variance in gene expression among donor cells and the hPSC lines shown in Fig. S2A. Red spheres indicate early passage sa-CB-derived MP-iPSCs (n=12, average passage=11.4); gold circles indicate hESCs (n=5, average passage=58.5); green circles indicate AdF-iPSCs (n=9, average passage=20.4); gray diamonds indicate donor fibroblasts; gray triangles indicate donor CB cells.