Fig. 4.

Complementary expression of mir-279/996 and Elav. (A) The bicistronic mir-279/996 locus, and a 16.6 kb knock-in reporter that contains all regulatory elements for genetic rescue (Sun et al., 2015), in which GFP replaces the mir-279 hairpin. (B) Wing imaginal disc carrying the mir-279-GFP transcriptional reporter shows ubiquitous expression, as well as upregulation in presumptive neural territories: the wing margin (WM) and dorsal radius (DR). (C) Magnification of the dorsal radius region co-stained for miR-279-GFP, the pan-sensory organ marker Cut, and Elav. The cells that upregulate mir-279-GFP are Cut⁺ Elav⁻ sensory organ cells. (D) Stage 14 embryo subjected to in situ hybridization for primary mir-279/996 transcripts and double labeling for Cut and Elav proteins. (E) Magnification of three lateral segments bearing PNS structures. In the embryo, Cut accumulates to a higher level in non-neural PNS cells. Endogenous mir-279/996 transcripts accumulate in Cut⁺ Elav⁻ cells. At least five imaginal discs or embryos were assayed for each genotype; representative images are shown.