Table 1.
Computer modeling accurately predicts STAT3 protein t1/2 in EBV cells generated from 8 of 8 AD-HIES patients
| Mutation category | Genotype (N)* | Stability prediction (stable = 0; unstable = 2) | Measured t1/2 ± SD (h) |
|---|---|---|---|
| None | wt/wt (N = 8) | Stable (0) | 25 ± 2 |
| Functional | R382W/wt (N = 4) | Stable (0.4) | 25.5 ± 3.3 |
| Structural | V463del/wt (N = 6) | Destabilizing (1) | 6.8 ± 4.8† |
| Y657S/wt (N = 6) | Significantly destabilizing (1.8) | 5.5 ± 4.1† | |
| T622I/wt (N = 4) | Destabilizing (0.6) | 18 ± 5.8‡ | |
| Structural-functional | R423Q/wt (N = 4) | Destabilizing (0.6) | 20 ± 2.3‡ |
| S611N/wt (N = 4) | Destabilizing (1.4) | 7 ± 3.6† | |
| N647D/wt (N = 4) | Destabilizing (0.8) | 18.5 ± 3‡ | |
| V637M/wt (N=4) | Destabilizing (1) | 5.3 ± 4.5† |
*
N = number of experiments performed with each EBV cell.
†
P < .0001 compared with WT.
‡
P < .05 compared with WT.