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. Author manuscript; available in PMC: 2017 Dec 20.
Published in final edited form as: Biomater Sci. 2016 Dec 20;5(1):41–45. doi: 10.1039/c6bm00692b

Fig. 4.

Fig. 4

Cells recognize and attach to protein-coated microbeads. Fluorescence and phase contrast images illustrate the self-assembly of MG-63, osteoscarcoma cells stained with calcein AM blue (blue, 10 µg/mL, AnaSpec Inc. Fremont, CA) in the presence of collagen-coated (green, pyrene-based dye, Sharpie) and uncoated (red, rhodamine-based dye, Sharpie) microbeads 1 hour (top) and 20 hours (bottom) after seeding. Spheroids containing cells alone (left column) assembled into compact structures over 20 hours. Spheroids containing cells and only coated microbeads (center-left column), only uncoated microbeads (center-right column), or both types of microbeads (right column) formed similar, compact spheroids but with different incorporation behavior of the microbeads.