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. Author manuscript; available in PMC: 2016 Dec 30.
Published in final edited form as: Mol Cell. 2010 Oct 8;40(1):99–111. doi: 10.1016/j.molcel.2010.09.021

Figure 2. ORCA Exists in Multiple Subcomplexes in the Cell.

Figure 2

(A) Glycerol gradient sedimentation analysis of ORCA complex on material immunoprecipitated with ORCA antibodies. The corresponding molecular weight markers are labeled. Note that ORCA, Orc2, and Orc3 cosediment in fractions 12–14.

(B) Association of ORCA with ORC in human cells. HeLa nuclear extract fractionated over a Superdex 200 gel filtration column and fractions analyzed for ORCA and various ORC subunits by immunoblot. Molecular weight markers are labeled on top of the panel. Note the multiple peaks of ORCA corroborating its existence in multiple subcomplexes.