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. Author manuscript; available in PMC: 2016 Dec 30.
Published in final edited form as: Mol Cell. 2010 Oct 8;40(1):99–111. doi: 10.1016/j.molcel.2010.09.021

Figure 4. ORCA Colocalizes with ORC at Heterochromatic Structures.

Figure 4

(A) IF with Orc2 pAb and HP1α antibodies after pre-extraction procedure in YFP-ORCA-expressing MCF7 cells reveals colocalization of ORCA with Orc2 and HP1α at heterochromatic regions.

(B) Triple immunolabeling with antibodies against ORCA, telomere binding protein Trf2, and centromere protein AnaC shows colocalization of ORCA with centromeric and pericentromeric heterochromatin in MCF7 cells.

(C) Immunostaining of Orc2 and HP1α in YFP-ORCA-expressing stable U2OS cells shows the complete overlap of ORCA and Orc2.

(D) Immunostaining of Trf2 and AnaC in YFP-ORCA-expressing stable U2OS cells shows colocalization of YFP-ORCA at telomeres but not at centromeres.

(E and F) Similar overlap of endogenous ORCA and YFP-Orc1 in U2OS cells at telomeres (E) but not at centromeres (F).

The scale bar represents 10 µm. See also Figure S3.