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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Oct 15;1861(2):418–430. doi: 10.1016/j.bbagen.2016.10.011

Figure 2. RP-amide HPLC of PNGase F-released O. dentatum N-glycans.

Figure 2

Pyridylaminated PNGase F-released N-glycans from male and female adults are shown annotated with the proposed structures (as confirmed by MS/MS and digestion data; see Figures 4-7 and the Supplementary Table); calibration is in terms of glucose units (g.u.). The PNGase F-released glycomes are dominated by oligomannosidic, α1,6-fucosylated, GalFuc-modified (late eluting) as well as phosphorylcholine-modified glycans.