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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Oct 15;1861(2):418–430. doi: 10.1016/j.bbagen.2016.10.011

Figure 5. MALDI-TOF MS analysis of two Hex3HexNAc2Fuc3 isomers.

Figure 5

(A and E) MS/MS of 2D-HPLC separated isomers of Hex3HexNAc2Fuc3 (m/z 1427 as [M+H]+; 6.8 and 8.0 g.u. from the PNGase A-released female glycome) with key fragments symbolically annotated; this is the most dominant multiply-fucosylated composition in the O. dentatum N-glycome. (B-D and F-I) MALDI-TOF MS before and after incubation with either Xanthomonas α1,2/3-mannosidase, hydrofluoric acid or Aspergillus β-galactosidase; the effect of these treatments is indicated with the dashed lines and alterations in MS/MS spectra (insets). The first isomer (B) corresponds to the sixth most dominant N-glycan in Haemonchus, whereas the second (F) is a minor structure in Pristionchus.