Fig. 3. Kinetics of 2-FPBA–CAL conjugation. (a) UV-vis absorption changes over time after mixing of 2-FPBA and CAL (pH 7, 10 μM each). (b) Example profile of the 2-FPBA–CAL conjugation kinetics. Data fitting yields the t1/2 values and the rate constants. Three separate measurements were carried out, from which the means of t1/2 and k2 were obtained together with their error values (shown in parentheses).
