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. Author manuscript; available in PMC: 2016 Dec 30.
Published in final edited form as: Pediatr Pulmonol. 2016 May 16;51(11):1142–1150. doi: 10.1002/ppul.23458

Fig. 3.

Fig. 3

Double immunofluorescence for co-localization of mesenchymal myogenic [fibroblast-specific protein (FSP)-1, α-SMA, or calponin] and epithelial (surfactant protein B or C) marker proteins. Representative lung section stained for the co-localization of α-SMA and FSP-1 (A), calponin and FSP-1 (B), SP-B and FSP-1 (C), and SP-C and FSP-1 (D) are shown. Fibroblast specific protein-1 staining co-localized with enhanced staining of mesenchymal proteins α-SMA and calponin, but not with epithelial proteins SP-B or SP-C. Images were taken at 20×. White arrows indicate the specific signals. N = 4.