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. 2016 Dec 30;11(12):e0169170. doi: 10.1371/journal.pone.0169170

Table 3. Effects of dietary CP levels or oscillating dietary CP on ruminal pH, total VFA concentration and individual VFA proportions in dual-flow continuous culture system.

Item1 Treatment, CP% SEM P-value
10% 12% 14% OSC 12% vs. OSC Linear Quadratic
pH 6.1 6.1 6.4 6.1 0.08 0.70 <0.01 0.12
Total VFA, mM 60.5 64.7 64.7 63.0 3.37 0.58 0.17 0.41
VFA, % total
    Acetate 35.6 34.5 30.9 38.8 2.99 0.29 0.25 0.72
    Propionate 28.9 31.2 33.2 28.9 2.38 0.41 0.14 0.95
    Butyrate 29.1 29.1 30.5 26.1 3.37 0.51 0.76 0.85
    Valerate 2.7 2.4 2.7 2.6 0.39 0.58 0.99 0.47
    Iso-Butyrate 0.1 0.4 0.5 0.5 0.51 0.23 0.70 0.36
    Iso-Valerate 3.1 2.5 2.3 3.0 0.54 0.38 0.19 0.64
BCVFA, mM 2.2 1.9 1.8 2.2 0.40 0.49 0.34 0.75
Acetate: propionate 1.3 1.1 1.0 1.4 0.12 0.07 0.05 0.83

1VFA = volatile fatty acids; BCVFA = Branched-chain VFA.