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. 2016 Dec 30;11(12):e0168832. doi: 10.1371/journal.pone.0168832

Table 2. Comparison of kinetic parameters of nuclease S1 wild type and its mutants N154S and N154A using ssDNA and RNA as a substrate.

Standard deviations are given.

	ssDNA				RNA
Nuclease S1	V_lim [ΔA_260nm/min/μg]	K_m [μg/μL]	V_lim/K_m [ΔA_260nmμL/min/μg²]	K_S [μg/μL]	V_lim [ΔA_260nm/min/μg]	K_m [μg/μL]	V_lim/K_m [ΔA_260nmμL/min/μg²]
Wild type	45 ± 3	0.14 ± 0.03	321	–	28 ± 3	0.16 ± 0.04	175
N154A	55 ± 7	0.32 ± 0.06	172	0.3 ± 0.1	17 ± 2	0.14 ± 0.03	121
N154S	16 ± 1	0.12 ± 0.02	133	–	23 ± 1	0.39 ± 0.04	59