Fig 4. G-003M restricts NF-κB nuclear translocation in mice jejunum exposed to gamma irradiation.

(A)G-003M was administered intra-muscularly into mice 1 hr prior to 9Gy whole body irradiation. After 6 hr, mice were sacrificed and intestinal epithelial nuclear and cytoplasmic protein extracts were prepared and assayed by immunoblotting for NF-κB, I-κBα expression. (B) Immunoflourescence (magnification; 40X) staining in intestinal epithelial cells (IEC) for NF-κB translocation was observed at 6 hr post-irradiation in mice injected with G-003M or DMSO 60 min before 9 Gy TBI. Corresponding nuclear: cytoplasmic ratios of NF-κB localisation in unstimulated control mice and 6 hr post irradiated (9 Gy) mice and mice pre-treated G-003M formulation 1 hr prior to 9 Gy whole body gamma radiation was quantified by IMAGE J software and expressed as mean±SD. 200 cells were randomly selected for scoring per animal. All the figures are representative results of three independent experiments.