Figure 3. Neural ensembles in IL reactive to S+ or S- following the activity-targeted disruption by Daun02.
Each of the four experimental groups, prepared and used for the Daun02 disruption (Figure 2B) and Post-disruption tests I (Figure 2C), was further randomly divided into three groups defined by the type of final Fos induction condition (S+, S- or No S+/S-). The rats were exposed to S+, S- or No S+/S- (control), then deeply anesthetized and euthanized. Brains were collected, sectioned (40 µm), processed for Fos immunohistochemistry. Fos-positive nuclei from sampling areas around the IL microinjection sites were quantified double-blindly. The average numbers of Fos-positive nuclei per mm2 were calculated for each rat, and used for statistical analyses. Yellow arrows represent typical Fos-positive nuclei. Group means of these average numbers (+SEM) are depicted. N = 7–9, each. *p<0.05–0.01 (vs. No active lever/light-cue).