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. 2016 Dec 3;5:e20718. doi: 10.7554/eLife.20718

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© 2016, Alcock et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — Strains contained the indicated amino acid substitutions in chromosomally encoded TatA or plasmid-encoded TatB or TatC. TatC^MTQ is a combination of the three substitutions M205A, T208A, and Q215A. TatAy is TatA with a C-terminal fusion to yellow fluorescent protein. (A) Transport activity of strains overproducing the Tat substrate CueO. Whole cell (W), spheroplast (S) and periplasm (P) fractions were subject to immunoblotting with antibodies against CueO or the cytoplasmic marker protein DnaK. m is the transported form of CueO from which the signal peptide has been removed and p the precursor protein. (B) Phase contrast images of the strains. `Δtat’ is the complete tat deletion strain DADE-A. (C) Membranes from the same strains were isolated and immunoblotted with a combination of TatB and TatC antibodies or with TatA antibodies.

DOI: http://dx.doi.org/10.7554/eLife.20718.012

Figure 5—figure supplement 1. — Strains contained the indicated amino acid substitutions in chromosomally encoded TatA or plasmid-encoded TatB or TatC. TatC^MTQ is a combination of the three substitutions M205A, T208A, and Q215A. TatAy is TatA with a C-terminal fusion to yellow fluorescent protein. (A) Transport activity of strains overproducing the Tat substrate CueO. Whole cell (W), spheroplast (S) and periplasm (P) fractions were subject to immunoblotting with antibodies against CueO or the cytoplasmic marker protein DnaK. m is the transported form of CueO from which the signal peptide has been removed and p the precursor protein. (B) Phase contrast images of the strains. `Δtat’ is the complete tat deletion strain DADE-A. (C) Membranes from the same strains were isolated and immunoblotted with a combination of TatB and TatC antibodies or with TatA antibodies.

DOI: http://dx.doi.org/10.7554/eLife.20718.012