Table 1.

Comparison of PSICOV predictions for Tat component contacts with those of other co-evolutionary methods. The programs PSICOV (Jones et al., 2012), CCMPRED (Seemayer et al., 2014), and FreeContact (mfDCA) (Hopf et al., 2012; Kaján et al., 2014) use methodologically distinct algorithms to calculate sequence co-evolution. Contacts predicted by all three of the co-evolutionary methods are colored green. Meta-PSICOV (Jones et al., 2015) aggregates information from the other three prediction methods as well as other sequence information in Stage 1, then weights by vicinity to other contacts in Stage 2. The table is divided to include the co-evolutionary contacts for TatA_All, TatA, TatB, TatA with TatA_basal, TatA with TatB_basal, and TatC-TatC contacts with separations greater than 20 Å. For the TatC-TatC contacts the three high-probabilty contacts identified by the three distinct methods suggest plausible contacts between the TM2-3 linker and residues at the C-terminal end of TM3 (green).

DOI: http://dx.doi.org/10.7554/eLife.20718.004

	PSICOV				FreeContact (mfDCA)				CCMPRED				Meta-PSICOV Stage 1				Meta-PSICOV Stage 2
	TMH	TatC	Score	Rank	TMH	TatC	Score	Rank	TMH	TatC	Score	Rank	TMH	TatC	Score	Rank	TMH	TatC	Score	Rank
TatAAll	12	202	0.71	51	12	202	1.46	108	12	202	0.30	28	12	202	0.75	22	12	202	0.46	112
	16	198	0.56	88	15	201	1.00	305	16	198	0.27	59	16	198	0.71	30	15	201	0.40	145
	15	201	0.5	109	16	198	0.95	335	15	201	0.23	117	15	201	0.64	49	16	198	0.34	191
	8	215	0.48	123	8	215	0.84	422	12	215	0.21	175	12	215	0.19	414	12	201	0.15	444
	12	215	0.38	189	12	215	0.75	549	18	21	0.19	313	15	198	0.14	609	12	198	0.08	771
	7	213	0.23	497	18	21	0.56	1010	11	212	0.18	323	18	21	0.14	611	8	215	0.05	1171
	8	208	0.22	578	8	208	0.53	1128	8	215	0.18	388	8	215	0.11	734	4	205	0.04	1332
	19	194	0.22	591	14	172	0.45	1577	15	198	0.18	400	8	208	0.09	860	8	205	0.04	1593
	5	208	0.22	619	8	205	0.44	1630	5	208	0.18	404	12	201	0.06	1072	5	208	0.03	1726
	18	21	0.21	670	5	126	0.43	1717	11	174	0.16	674	12	198	0.06	1083	15	198	0.03	1897
	11	25	0.2	768	15	198	0.42	1761	8	208	0.15	753	14	203	0.05	1175	8	208	0.03	1963
	8	205	0.19	772	17	85	0.42	1851	4	205	0.15	793	4	205	0.05	1176	11	202	0.03	2044
	8	166	0.19	855	5	124	0.39	2105	17	227	0.15	802	5	208	0.04	1367	8	198	0.02	2299
	12	198	0.18	911	5	132	0.38	2131	19	194	0.15	960	11	25	0.04	1424	15	202	0.02	2569
	17	227	0.17	1003	12	194	0.33	2870	8	214	0.15	979	11	174	0.04	1544	4	208	0.02	2846
	15	198	0.17	1150	12	75	0.32	2995	12	198	0.15	1036	15	219	0.03	1574	8	202	0.02	3287
	12	201	0.16	1183	12	198	0.32	3008	12	201	0.14	1158	10	25	0.03	1607	4	206	0.01	3437
TatA	12	202	0.71	18	12	202	1.81	16	12	202	0.21	10	12	202	0.72	12	12	202	0.46	91
	16	198	0.55	39	16	198	1.12	124	16	198	0.19	15	16	198	0.72	13	9	206	0.27	229
	14	216	0.38	122	12	215	1.06	155	9	206	0.14	49	9	206	0.40	82	16	198	0.27	230
	16	82	0.37	133	5	136	0.91	246	15	163	0.14	67	15	163	0.35	98	4	206	0.18	378
	9	206	0.34	162	5	210	0.86	287	3	205	0.14	72	15	202	0.29	137	9	202	0.11	630
	5	162	0.29	262	15	163	0.86	297	12	215	0.14	74	16	82	0.28	146	8	206	0.09	830
	5	136	0.29	270	8	212	0.77	408	13	197	0.14	79	15	201	0.24	184	5	210	0.09	835
	9	39	0.28	290	19	170	0.74	470	16	82	0.14	86	19	20	0.20	271	15	202	0.09	866
	12	215	0.26	339	15	202	0.72	530	2	219	0.13	121	12	215	0.18	317	18	232	0.09	880
TatB	18	21	0.83	7	18	21	2.19	6	18	21	0.23	9	12	198	0.72	12	12	198	0.61	41
	12	215	0.60	30	12	215	1.45	22	12	198	0.18	14	12	202	0.63	23	12	202	0.47	97
	12	202	0.56	36	12	198	1.39	24	18	24	0.17	16	18	21	0.61	29	18	21	0.41	125
	20	185	0.46	65	12	202	1.13	63	12	202	0.16	17	18	24	0.53	47	18	24	0.32	194
	12	198	0.39	110	19	13	1.04	89	12	215	0.16	21	12	215	0.49	55	5	212	0.20	350
	5	213	0.36	127	5	212	0.97	117	17	227	0.14	71	19	13	0.39	79	12	215	0.20	355
	7	204	0.35	141	5	126	0.87	164	14	167	0.14	72	14	24	0.25	175	4	206	0.16	462
	5	212	0.28	281	18	24	0.81	221	5	213	0.13	88	19	198	0.22	225	5	208	0.14	529
TatA with TatAbasal	12	202	0.75	28	12	202	1.85	26	16	198	0.31	10	16	198	0.80	8	12	202	0.47	94
	16	198	0.70	35	16	198	1.26	62	12	202	0.30	11	12	202	0.77	14	16	198	0.43	111
	15	201	0.60	58	8	215	1.01	130	15	201	0.22	40	15	201	0.68	36	15	201	0.39	136
	8	215	0.58	64	15	201	1.01	131	11	212	0.17	126	8	215	0.18	309	12	201	0.08	644
	19	194	0.38	141	12	215	0.69	378	8	215	0.16	143	14	203	0.14	419	8	215	0.06	832
	8	205	0.27	309	5	136	0.67	426	12	215	0.16	159	8	208	0.09	605	4	205	0.06	852
TatB with TatAbasal	18	21	0.48	72	18	21	1.42	44	18	21	0.23	19	12	198	0.65	36	12	198	0.48	94
	12	198	0.46	79	12	198	0.99	173	12	198	0.19	37	12	202	0.56	55	4	206	0.43	119
	12	202	0.38	123	12	202	0.84	281	12	202	0.19	39	18	21	0.56	56	18	21	0.28	214
	20	185	0.37	134	12	215	0.76	383	18	24	0.18	44	15	201	0.34	122	12	202	0.28	218
	12	215	0.33	180	15	201	0.73	427	14	167	0.17	69	4	206	0.28	168	4	207	0.28	225
	5	213	0.30	252	5	212	0.68	514	5	213	0.17	88	18	24	0.27	175	4	205	0.16	415
TatC-TatC	64	134	0.79	33	27	199	2.04	39	64	134	0.28	51	64	134	0.6	62	64	134	0.64	60
	71	140	0.77	41	65	137	1.72	62	71	140	0.26	75	71	140	0.54	84	27	199	0.47	108
	65	137	0.64	69	71	140	1.63	72	65	137	0.21	191	27	199	0.48	103	65	137	0.3	227
	28	203	0.51	10864	64	134	1.51	93	113	228	0.19	319	65	137	0.39	150	64	137	0.24	283
	72	217	0.42	155	64	157	1.05	284	67	140	0.18	345	28	203	0.37	162	33	167	0.23	289