. 2016 Dec 3;5:e20718. doi: 10.7554/eLife.20718

Table 3.

Plasmids used in this study.

DOI: http://dx.doi.org/10.7554/eLife.20718.026

Plasmid name	Abbreviation	Description	Reference
pTH19cr		Low copy number pSC101-derived replicon. Chl^r.	(Hashimoto-Gotoh et al., 2000)
p101C*TatBC	pBC	pTH19cr derivative. Expression of tatBC from the tatA promoter with a modified RBS.	(Alcock et al., 2013)
p101C*BC M205R	pBC^M205R	p101CTatBC tatC-met205arg*	This work
p101C*BC Q215A	pBC^Q215A	p101CTatBC tatC-gln215ala*	This work
p101C*BC MTQ	pBC^MTQ	p101CTatBC tatC- met205ala- thr208ala- gln215ala*	This work
p101C*BC M205A	pBC^M205A	p101CTatBC tatC- met205ala*	This work
p101C*BC T208A	pBC^T208A	p101CTatBC tatC- thr208ala*	This work
p101C*BC TQA	pBC^T208A,Q215A	p101CTatBC tatC- thr208ala- gln215ala*	This work
p101C*BC FEA	pBC^FEA	p101CTatBC tatC- Phe 94ala- glu103ala*	(Alcock et al., 2013)
p101C*BC FEAMR	pBC^{FEA, M205R}	p101CBC FEA tatC-met205arg*	This work
p101C*BC FEAQA	pBC^{FEA, Q215A}	p101CBC FEA tatC-gln215ala*	This work
p101C*BC FEAMTQ	pBC^{FEA, MTQ}	p101CBC FEA tatC- met205ala- thr208ala- gln215ala*	This work
p101C*BC FEAMA	pBC^FEA,M205A	p101CBC FEA tatC- met205ala*	This work
p101C*BC FEATA	pBC^FEA,T208A	p101CBC FEA tatC- thr208ala*	This work
p101C*BC FEATQA	pBC^{FEA,T208A, Q215A}	p101CBC FEA tatC- thr208ala- gln215ala*	This work
p101C*BC E8A	pB^E8AC	p101CTatBC tatB-glu8ala*	This work
p101C*BC EAFEA	pB^E8AC^FEA	p101CBC FEA tatB-glu8ala*	This work
p101C*BC EFM	pB^E8AC^{FEA, MTQ}	p101CBC FEAMTQ tatB-glu8ala*	This work
p101CTatC		pTH19cr derivative expressing tatC from the tatA promoter	This work
p101C*TatC	pC	p101CTatC with a modified RBS and GTG start codon.	This work
p101C*TatC FEA	pC^FEA	p101CTatC tatC- Phe 94ala- glu103ala*	This work
p101C*BC V18W	pB^V18WC	p101CTatBC tatB-val18trp*	This work
p101C*BC L21W	pBC^L21W	p101CTatBC tatC-leu21trp*	This work
p101C*BC VLW	pB^V18WC^L21W	p101CTatBC V18W tatC-leu21trp*	This work
pTat101 cys less		Very low copy number vector expressing tatABC^{C23A,C33A,C179A,C224A} from the tat promoter. Kan^r.	(Cléon et al., 2015)
p101C*BC cys less	pBC cys^-	p101CTatBC tatC-cys23ala-cys33ala-cys179ala-cys224ala*	This work
p101C*BC V18C	pB^V18CC	p101CBC cys less tatB-val18cys*	This work
p101C*BC L21C	pBC^L21C	p101CBC cys less tatC-leu21cys*	This work
p101C*BC 17C 21C	pB^L17CC^L21C	p101CBC L21C tatB-leu17cys*	This work
p101C*BC 18C 21C	pB^V18CC^L21C	p101CBC L21C tatB-val18cys*	This work
p101C*BC 17C 22C	pB^L17CC^N22C	p101CBC cys less tatB-leu17cys tatC-asn22cys*	This work
p101C*BC 18C 22C	pB^V18CC^N22C	p101CBC V18C tatC-asn22C*	This work
p101C*BC 17C 23C	pB^L17CC^C23C	p101CBC cys less tatB-leu17cys tatC-ala23cys*	This work
p101C*BC 18C 23C	pB^V18CC^C23C	p101CBC V18C tatC-ala23cys*	This work
p101C*BC 17C 24C	pB^L17CC^I24C	p101CBC cys less tatB-leu17cys tatC-ile24cys*	This work
p101C*BC 18C 24C	pB^V18CC^I24C	p101CBC V18C tatC-ile24cys*	This work
p101C*BC 17C 25C	pB^L17CC^I25C	p101CBC cys less tatB-leu17cys tatC-ile25cys*	This work
p101C*BC 18C 25C	pB^V18CC^I25C	p101CBC V18C tatC-ile25cys*	This work
pUnitat2		Expression of tatABC_his under the control of a T5 promoter	(McDevitt et al., 2005)
pUT2DH		Expression of tatABC under the control of a T5 promoter.	This work
pUT2DH FEA		pUT2DH tatC- Phe 94ala- glu103ala	This work
pUT2DH FEAMR		pUT2DH FEA tatC-met205arg	This work
pUT2DH EAFEA		pUT2DH FEA tatB-glu8ala	This work
pQE80-CueO		Synthesis of E. coli CueO with a C-terminal his₆ tag.	(Leake et al., 2008)
pKSUniA		tatA under control of the tat promoter in pBluescript KS⁺	(Koch et al., 2012)
pKSUniA Q8A		pKSUniA tatA-gln8ala	This work
pRS552		lambda attachment site shuttle vector	(Simons et al., 1987)
p552TatA Q8A		pRS552 carrying P_tatAtatA^Q8A	This work