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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Virology. 2016 Nov 14;501:35–46. doi: 10.1016/j.virol.2016.11.001

Fig. 5.

Fig. 5

Comparison of rLCMV/WT and rLCMV/CD virion particles. rLCMV/WT and rLCMV/CD virions were purified from infected (moi=0.01) BHK-21 cells at 72 h p.i. by ultracentrifugation through an 20% (wt/vol) sucrose cushion. Morphology of rLCMV/WT (A) and rLCMV/CD (B) was assessed by TEM using negative straining. Same viral preparations were surface stained with the LCMV GP1-specific monoclonal antibody 36.1 and counterstained with 12-nm-gold-labeled goat anti-mouse IgG antibody (C). The number of gold particles/virion was determined for rLCMV/WT (N=19) and rLCMV/CD (N=32) immune stained viruses (D). Representative images are shown. Scale bar=100 μm.