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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Virology. 2016 Dec 2;501:127–135. doi: 10.1016/j.virol.2016.11.011

Figure 7.

Figure 7

Reduced viral nucleocapsid association with lipid rafts after cholesterol depletion. Cells untreated or treated with drugs were infected with mock, SeV or hPIV1. Membrane floatation assay was performed to quantitate the raft-associated NP in each fraction. Caveolin-1 and SeV NP were detected by immunoblotting. Cells infected with hPIV1 were radiolabeled with 35S-Met/Cys and labeled NPs in each fraction were immunoprecipitated and resolved by SDS-PAGE. The numbers show percentage of the proteins recovered from raft (1–4) and non-raft (7–9) fractions.