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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Acta Physiol (Oxf). 2016 Jul 20;219(2):521–536. doi: 10.1111/apha.12742

Fig. 3. Electrophysiological properties of MDCK I TetOff cells expressing wild-type or D65N claudin-2.

Fig. 3

(a) Conductance and (b) Na+ permeability of two different clones of wild-type (WT) and mutated (D65N) claudin-2 (n=7–14). The upper panel in (A) is a Western blot (from Fig. 2b) showing claudin-2 protein expression in each clone (left lane, Dox+; right lane, Dox−). Permeability was derived from measurement of dilution potentials and calculated by means of the Goldman-Hodgkin-Katz equation. As expected, a strong difference in conductance and permeability could be observed between induced (Dox−) and uninduced (Dox+) cells and also between cells expressing wild-type and mutated claudin-2. (*** p < 0.001 vs. Dox+, ### p < 0.001 vs. WT#12 and WT#15).